Glioblastoma Multiforme and Angiogenesis: A Clinicopathological and Immunohistochemistry Approach

Martha Lilia Tena-Suck, Miguel Angel Celis-Lopez, Miguel Angel Collado-Ortiz, Manuel Castillejos-Lopez, Mariana Tenorio-Serralta


Background: Glioblastomas multiforme (GBMs) are histologically heterogeneous tumors derived from highly vascularized glia. This microvascular proliferation is a biological key to diagnosis.

Methods: The purpose of this study was to analyze the immunohistochemistry features of these lesions, looking for commonly expressed antibodies such as vimentin, nestin, glial fibrillary acidic protein (GFAP), GFAP delta (GFAP-δ), vascular endothelial growth factor (VEGF), VEGF receptor 2 (VEGF-R2), VIII factor, CD31, CD34 and CD15 in 80 cases of GBM patients with a 15-month follow-up. Microvascular density (MVD) and Ki-67 labeling index (LI) were performed.

Results: Eighty patients’ GBMs were surgically removed in 1 year with a 15-month follow-up, including 64 men (80%) and 16 women (20%). The age range was 37 - 87 years (mean age 58.36 ± 9.929 years). Seventeen (21%) cases were less than 50 years old (P = 0.003). The range of age in men was 37 - 71 years (median age 56 years) and in women was 39 - 86 years (median age 58 years). Thirty-five (44%) cases had secondary tumors and 45 (56%) had primary tumors. Survival time ranged between 14 and 60 weeks (mean 44.40 ± 16.26 weeks). Inflammation and necrosis were more common in primary than in secondary, also with one showing higher MVD index for VEGF, VEGF-R2, CD31 and CD34.

Conclusion: Expression of nestin, vimentin, GFAP-δ, VEGF, and VEGF-R2 peritumoral cells has bad prognosis in GBM.

J Neurol Res. 2015;5(3):199-206



Angiogenesis; Glioblastoma multiforme; Immunohistochemistry; VEGF; VEGF-R2; Nestin; Vimentin; CD34; MVD

Full Text: HTML PDF

Browse  Journals  


Journal of Clinical Medicine Research

Journal of Endocrinology and Metabolism

Journal of Clinical Gynecology and Obstetrics


World Journal of Oncology

Gastroenterology Research

Journal of Hematology


Journal of Medical Cases

Journal of Current Surgery

Clinical Infection and Immunity


Cardiology Research

World Journal of Nephrology and Urology

Cellular and Molecular Medicine Research


Journal of Neurology Research

International Journal of Clinical Pediatrics



Journal of Neurology Research, biannually, ISSN 1923-2845 (print), 1923-2853 (online), published by Elmer Press Inc.                     
The content of this site is intended for health care professionals.
This is an open-access journal distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, which permits unrestricted
non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Creative Commons Attribution license (Attribution-NonCommercial 4.0 International CC-BY-NC 4.0)

This journal follows the International Committee of Medical Journal Editors (ICMJE) recommendations for manuscripts submitted to biomedical journals,
the Committee on Publication Ethics (COPE) guidelines, and the Principles of Transparency and Best Practice in Scholarly Publishing.

website:   editorial contact:
Address: 9225 Leslie Street, Suite 201, Richmond Hill, Ontario, L4B 3H6, Canada

© Elmer Press Inc. All Rights Reserved.

Disclaimer: The views and opinions expressed in the published articles are those of the authors and do not necessarily reflect the views or opinions of the editors and Elmer Press Inc. This website is provided for medical research and informational purposes only and does not constitute any medical advice or professional services. The information provided in this journal should not be used for diagnosis and treatment, those seeking medical advice should always consult with a licensed physician.